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At the normal pH of plasma (7.4) and, therefore, of the glomerular filtrate, we find that about 80% of the phosphate is in the base (divalent) form and 20% is in the acid (monovalent) form. As the tubular fluid is acidified in the collecting ducts, most of the base form combines with secreted hydrogen ions. By the time the minimum intratubular pH of 4.4 is reached, virtually all the base (HPO422) has been converted to acid (H2PO42). Therefore, secreted hydrogen ions that combined with the base form are excreted, and the bicarbonate that was generated intracellularly in the process enters the blood. How much phosphate is available for this process The amount is somewhat variable, depending on a number of factors (see 10), but a typical plasma concentration is about 1 mmol/L, of which about 90% is free (the rest being loosely bound to plasma proteins). At a GFR of 180 L/day, the total filtered load of phosphate is about160 mmol/day. The fraction reabsorbed is also variable: from 75% to 90%. Thus, unreabsorbed divalent phosphate available for buffering is roughly 40 mmol/day. In other words, the kidneys can excrete hydrogen ions, using the phosphate buffer system, at a rate of about 40 mmol/day. However, the availability of phosphate cannot be easily upregulated to increase acid excretion. There are other organic buffers in the urine, and under certain conditions these may appear in the tubular fluid in sufficient quantities to allow them also to act as important buffers. A particularly important example is a patient with uncontrolled diabetes mellitus. Because of metabolic processes that result from insulin deficiency, such a patient may become extremely acidotic due to the excess production of acetoacetic acid and -hydroxybutyric acid. At normal plasma pH,

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